Diane is one of Jeff's puppets. She is an alcoholic and possibly a black widow bride. She admits to killing her second husband by causing him to have a stroke. She was seen in the 2010 film Dinner for Schmucks. She is Jeff's only female puppet.
In Minding the Monsters, she was confirmed retired as her name was on a tombstone in the opening sequence. She never appeared in a Comedy Central special. Jeff said the reason why she won't be used again is because, despite all the women he lives with, he can't think like a women.